Dioclea virgata wikispecies wikispecies, free species. All these activities were reversed by binding the lectin to glucose or. Antiglioma properties of dvl, a lectin purified from dioclea. Plants mostly herbs rarely shrubs, all leaves basal, simple, stipulate. Oxalis violacea violet wood sorrel pfaf plant database. Jun 18, 2014 this page was last edited on 3 september 2017, at 07. International journal of biological macromolecules 120, 566577, 2018. Pdf acute kidney injury aki is characterized by rapid and potentially reversible decline in renal.
Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Berman, rutgers board of governors professor of chemistry and. Please note that this is a trial program and may end. The lectins are from canavalia brasiliensis, canavalia bonariensis, cratylia floribunda, dioclea rostrata, dioclea virgata, dioclea violacea, and dioclea guianensis. Dioclea state, a medieval serb state of duklja, in southeastern part of modern montenegro dioclea in praevalitana, an ancient roman and byzantine city in the province of praevalitana, near modern podgorica in montenegro. Dioclea violacea lectin ameliorates oxidative stress. The histamine releasing properties of glucose mannosespecific lectins isolated from brazilian beans was examined. The structural basis of the ph dependency of the dimertetramer transition exhibited by brindas type ii diocleinae lectins was investigated by equilibrium sedimentation and x. All structured data from the file and property namespaces is available under the creative commons cc0 license. Antinociceptive activity of lectins from diocleinae seeds. Diocleinae lectins administered per oral route in mice inhibited the abdominal constrictions induced by acetic acid. This lectin exhibits various pharmacological properties. The lectins included those from canavalia brasiliensis, cratylia floribunda, dioclea rostrata, dioclea virgata, dioclea violacea, and dioclea guianensis.
The record derives from ildis data supplied on 20100714 which reports it as an accepted name record 16815. Plantderived lectins as potential cancer therapeutics and. Isolation and characterization of novel lectins from. Effect of leguminous lectins on the growth of rhizobium. Antiglioma properties of dvl, a lectin purified from dioclea violacea apm nascimento, jl knaut, dk rieger, iav wolin, ia heinrich, j mann. Crystal structure of dioclea violacea lectin and a. Pdf dioclea violacea lectin ameliorates oxidative stress and renal.
Dioclea is a genus of flowering plants in the pea family, fabaceae, that is native to the americas. Concanavalin a as an inducer of human lymphocyte mitogenic. Acute kidney injury aki is characterized by rapid and potentially reversible decline in renal function. The seeds of these legumes are buoyant drift seeds, and are dispersed by rivers. Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury flavia ps freitas, 1 marcella l porto, 1 camilla p tranhago, 1 rogerio piontkowski, 2 emilio c miguel, 3 thaiz bar miguel, 3 jorge l martins, 4 kyria s nascimento, 3 camille m balarini, 5 benildo s cavada, 3. Differences in structure of lectins were found to be re. In this study, the dglucose dmannosebinding lectins from seeds of dioclea megacarpa, d. Acute kidney injury, ischemia reperfusion, renal function, plant lectin, dioclea violacea. Ch tshape interaction with histidine explains binding of. The primary sequence, as determined by tandem mass spectrometry, revealed a protein with 237 amino acids and 81% of identity with cona. Our data demonstrate that dvl administration in ir animals increases glomerular filtration rate, renal plasma and blood flow, while, at the same time, it diminishes renal vascular resistance. Caesalpinia violacea is a fastgrowing tree with the capability to grow and establish in poor clay, stony and sandy soils. Lectin from the seeds of dioclea sclerocarpa dsl was purified in a single step by affinity chromatography on a sephadex g50 column. The galactose specific lectin leca mediates biofilm formation in the opportunistic pathogen p.
Plants have escaped from cultivation and, in cuba, the species is listed as invasive and as one of the more noxious invasive plants. Discover lifes page about the biology, natural history, ecology, identification and distribution of dioclea wilsonii wilsons clusterpea discover life. A bulbous, stemless perennial typically growing 48 tall in which the longstemmed leaves and longer, leafless flower stalks rise directly from the bulb. Access is allocated within two business days of purchase and lasts one year. Dioclea violacea lectin ameliorates oxidative stress and. Briefly, the lectins were extracted from airdried ground seeds collected in fortalezace, brazil, and defatted with nhexane. Pdf dioclea violacea martius, ex bentham, contain a lectin that could. Files are available under licenses specified on their description page. The integrated taxonomic information system itis provides authoritative taxonomic information on dioclea reflexa, as well as other plants, animals, fungi, and microbes of north america and the world. Multiple compounds assessed as novel potential anticancer drugs derive from natural sources, including microorganisms, plants, and animals. A lectin from dioclea violacea interacts with midgut. Less potency and efficacy was observed forcanavalia maritima, dioclea guianensis. The generality of this interaction was tested in a diverse. Briefly, the seeds were ground into a fine powder and stirred with 0.
Lectin was extracted from the seeds of dioclea violacea dvl by affinity chromatography as previously described. Thermodynamic binding studies of lectins from the diocleinae. Read structural analysis of a dioclea sclerocarpa lectin. Enjoy unrestricted access to all content and features, including. Study on the vasorelaxant properties of dioclea lectins, international journal of biological macromolecules on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. In vitro inhibition of streptococci binding to enamel. Contents the international journal of biochemistry cell. These results indicate that dvl can be considered a new therapeutic approach for akiinduced kidney injury.
Flavia ps freitas physiological sciences graduate program, health sciences center, federal university of espirito santo vitoria, es, brazil. Less potency and efficacy was observed forcanavalia maritima, dioclea guianensis, anddioclea violacea while very low. Dioclea in phrygia, an ancient city and former bishopric in phrygia asia minor. Dioclea violacea lectin dvl is a protein that recognizes and binds primarily to manglc saccharides present in cell surface glycans 17. Lectin from dioclea violacea induces autophagy in u87 glioma cells ana paula m. Determinants of quaternary association in legume lectins.
A lectin from dioclea violacea interacts with midgut surface. Lectins, a group of highly diverse proteins of nonimmune origin with carbohydratebinding abilities, have been detected in. Contents the international journal of biochemistry cell biology. Susceptibility and refractoriness to leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. The plant list includes a further 8 scientific plant names of infraspecific rank for the genus dioclea. We do not intend the plant list to be complete for names of infraspecific rank. The journal publishes papers by leading scientists from all over the world that report on advances in the understanding of proteins in the broadest sense. Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury. The generality of this interaction was tested in a. Seven other lectins of the subtribe diocleinae from different genera and different species scheme i have recently been described. Two lectins were isolated from canavalia ensiformis and dioclea grandiflora seeds. It is invading natural habitats where it is outcompeting native species. Molecules free fulltext effect of leguminous lectins on.
See status, confidence level, source for definitions. Dvl is a legume lectin with affinity for glucosemannose, and it belongs to the subtribe diocleinae, similar to cona. Thecanavalia brasiliensis, dioclea rostrata, anddioclea virgata lectins induced histamine release in rat peritoneal mast cells similar to concanavalin a. Text is available under the creative commons attributionsharealike license. Diocleinae lectins are a group of proteins with conserved. Ontario, canada south to georgia and westward to texas, oklahoma, kansas and nebraska known invasiveness. Fluorescence micrography was employed to visualize the ability of fitc. Cancer remains a global health challenge, with high morbidity and mortality, despite the recent advances in diagnosis and treatment. The lectins used in this work were extracted from canavalia ensiformis, canavalia brasiliensis, dioclea violacea, dioclea grandiflora, cratylia floribunda and vatairea macrocarpa.
Lectins from cratylia floribunda cfl, dioclea violacea dvl, and canavalia gladiata cgl were purified as previously reported 1921. This page was last edited on 3 september 2017, at 07. Crystallization kits were used to obtain protein crystals and the better condition was 4m sodium formate. J f m a m j j a s o n d a tuftforming bulbous plant with straplike greygreen leaves that are strongly garlic scented when bruised. The international legume database and information service or ildis tropicos link. The lectin extracted from the seed of dioclea violacea, dvl, was purified for the first time in 1996 by moreira et al. In the present study, we evaluated the effects of dioclea violacea lectin administered prior to ischemiareperfusion irinduced acute kidney injury aki. The sdspolyacrylamide gel electrophoresis patterns of the subunit. The species is hermaphrodite has both male and female organs and is pollinated by insects. Resolution of the three dimensional structure of lectin. The effects of the lectin of dioclea violacea dvl, purified by moreira et al.
Ch tshape interaction with histidine explains binding. Crystal structure of dioclea violacea lectin and a comparative study of vasorelaxant properties with dioclea rostrata lectin. All of the lectins exhibited specificity for man and glc residues, but much higher affinities for the branched chain trimannoside, 3,6di o. The dioclea violacea lectin dviol was purified on a sephadex g50 column and crystallized in the presence of the xmannose by the vapor diffusion method. Quaternary structure of dioclea grandiflora lectin. Lectins, a group of highly diverse proteins of nonimmune origin with carbohydratebinding. Ipni nomenclature info for dioclea reflexa nomenclatural information about dioclea reflexa is provided by the international plant names index ipni. Diocleinae lectins are a group of proteins with conserved binding. It is in flower from may to august, and the seeds ripen from july to september. Jan 01, 2016 read structural analysis of a dioclea sclerocarpa lectin. Antiglioma properties of dvl, a lectin purified from. Protein science, the flagship journal of the protein society, serves an international forum for publishing original reports on all scientific aspects of protein molecules.
Dioclea violacea lectin ameliorates oxidative stress and renal. Dioclea kunth show all show tabs dioclea general information. Dioclea or diocleia, and also dioklea or diokleia may refer to. Gel filtration produced a fraction corresponding to con a or d. The percentage of the lectins antinociception varied from 61% for canavalia grandiflora congf to 20% for dioclea violacea. Dec 15, 2015 dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in an experimental model of acute kidney injury. Structurally, dvl appears in tetrameric form in neutral ph and dimeric form in more acidic ph. Crystal structure of dioclea violacea lectin and a comparative study. Violet lespedeza lespedeza violacea propagation protocol.
Histamine release induced by glucose mannosespecific. In this study lectins purified from the seeds of dioclea violacea dvl and dioclea. Briefly, the lectins were extracted from airdried ground seeds collected in fortalezace, brazil, and defatted with n. Protein science aims to unify this field by cutting across. Manual adjustments in coot were made after each refinement step and an x man. Leishmaniasis is a vectorborne disease transmitted by phlebotomine sand fly. Pdf isolation and partial characterization of a lectin from seeds of.